Product Name:
Human Basic Salivary Proline Rich Protein 2 (PRB2) ELISA Kit
Cat.#:
JL30601
Regulatory Status:
Alternative:
PRPPRB1; Ps; cP7; Proline-Rich Protein BstNI Subfamily 2; Con1 glycoprotein; Basic peptide P-F
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.06ng/mL
Specificity:
This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed.
Sample Type:
saliva and other biological fluids
Assay Procedure:

Research Area:
Immunology
Product Datasheets
| PRB2
Basic Salivary Proline Rich Protein 2,also known as PRB2 or Recombinant Basic Salivary Proline Rich Protein 2 (Recombinant PRB2), is a type of recombinant protein. Recombinant Basic Salivary Proline Rich Protein 2 (PRB2) is a protein derived from human saliva, characterized by its high proline content. It belongs to the family of salivary proline-rich proteins, which play important roles in various biological processes, such as maintaining the stability of saliva and protecting oral tissues.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0.15 | 0 |
OD | 2.12 | 1.58 | 1.14 | 0.77 | 0.53 | 0.35 | 0.24 | 0.08 |
Corrected OD | 2.04 | 1.5 | 1.06 | 0.69 | 0.45 | 0.27 | 0.16 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human PRB2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Saliva(n=8) | 84-101 | 96 |
| Linearity
Samples were spiked with high concentrations of Human PRB2 and diluted with Universal Diluent to produce samples with values within the range of the assay.Saliva(n=4) | ||
1 : 2 | Range (%) | 84-95 |
Average (%) | 91 | |
1 : 4 | Range (%) | 89-103 |
Average (%) | 94 |






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